H. pylori can be cultured in a "candle jar" on blood
agar or "chocolate agar". These are common media, which
can be obtained from any microbiology laboratory. Remember that
H. pylori likes to have lots of moisture and also likes to
have reduced amounts of oxygen with extra carbon dioxide. Typically
this can be obtained in a "candle jar". A candle jar is
made by placing wet paper towels in the bottom of an airtight container.
The petri-dishes are placed on top of the wet towels, and above
all this a candle is placed and set alight before closing the lid.
The candle uses about half of the oxygen in the jar and replaces
it with carbon dioxide. Usually H. pylori will be detected
after 3 days of culture in this setting.
It must be remembered that H. pylori is a pathogen, which
means that it can potentially cause an infection in humans or animals.
Because of this it is strictly controlled in the laboratory and
the usual precautions have to been taken when handling this type
of bacteria. It has also been named as a potential carcinogen as
its connection with gastric ulceration has been shown to link to
gastric cancers and as such should be strictly controlled in the
non-clinical laboratory setting.